The Rat Desmin CLIA Kit (RTES00178) from Assay Genie is a highly sensitive and specific assay designed for the accurate detection of desmin levels in rat samples. Desmin is a key protein involved in maintaining the structure and function of muscle cells, making it an important biomarker for studying muscle diseases and disorders.This kit provides researchers with reliable and reproducible results, making it ideal for a wide range of research applications.
Whether you are studying muscle regeneration, muscle degeneration, or muscle-related diseases, the Rat Desmin CLIA Kit can help you gain valuable insights into the role of desmin in these processes.Don't miss out on this opportunity to advance your research with the Rat Desmin CLIA Kit from Assay Genie. Order yours today and take your research to the next level!
Product Name:
Rat Des (Desmin) CLIA Kit
SKU:
RTES00178
Target:
Rat Des (Desmin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat Des. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat Des and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat Des, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat Des. You can calculate the concentration of Rat Des in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-109
100-112
103-115
Average (%)
100
106
109
1:4
Range (%)
100-118
103-120
92-106
Average (%)
108
110
99
1:8
Range (%)
100-116
99-113
92-105
Average (%)
108
106
97
1:16
Range (%)
100-115
96-109
101-116
Average (%)
107
102
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-108
102
EDTA plasma (n=5)
90-104
95
Cell culture media (n=5)
90-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
210.59
378.75
1758.2
201.91
374.39
1617.35
Standard deviation
26.26
36.93
144.0
25.62
35.04
151.71
C V (%)
12.47
9.75
8.19
12.69
9.36
9.38
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat Des concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat Des in samples. No significant cross-reactivity or interference between Rat Des and analogues was observed.
