Rat DHEA-S (Dehydroepiandrosterone Sulfate) ELISA Kit
The Rat DHEA-S (Dehydroepiandrosterone Sulfate) ELISA Kit is a specialized assay designed for the quantitative measurement of dehydroepiandrosterone sulfate levels in rat biological samples. Dehydroepiandrosterone sulfate, commonly known as DHEA-S, is a steroid hormone that serves as a precursor to various sex hormones, including testosterone and estrogen. DHEA-S is produced by the adrenal glands and plays a crucial role in regulating hormone balance and metabolism. DHEA-S levels are often used as markers of adrenal function and overall endocrine health. Abnormal DHEA-S levels have been associated with conditions such as adrenal insufficiency, hormonal imbalances, and certain diseases.
By measuring DHEA-S concentrations, researchers and clinicians can gain insights into adrenal function, hormonal status, and potential health risks related to endocrine disorders. The Rat DHEA-S ELISA Kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results. Manufactured under strict quality control standards, this kit provides reliable performance and user-friendly protocols, making it an excellent choice for studying adrenal function, endocrine health, and hormonal balance in rat models.
Product Name:
Rat DHEA-S (Dehydroepiandrosterone Sulfate) ELISA Kit
SKU:
AEES00377
Target:
Rat DHEA-S (Dehydroepiandrosterone Sulfate)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with DHEA-S. During the reaction, DHEA-S in the sample or standard competes with a fixed amount of DHEA-S on the solid phase supporter for sites on the Biotinylated Detection Ab specific to DHEA-S. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of DHEA-S in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-103
91-107
87-98
Average (%)
94
98
93
1:4
Range (%)
83-98
90-105
97-114
Average (%)
89
98
105
1:8
Range (%)
86-102
88-102
97-114
Average (%)
93
94
104
1:16
Range (%)
86-99
86-97
95-112
Average (%)
93
92
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
94
EDTA plasma (n=5)
86-99
91
Cell culture media (n=5)
86-100
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.9
174.4
936.3
89.9
166.7
1012.3
Standard deviation
5.1
7.5
36.5
5.2
7.3
48.6
C V (%)
5.32
4.3
3.9
5.78
4.38
4.8
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of DHEA-S concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes DHEA-S in samples. No significant cross-reactivity or interference between DHEA-S and analogues was observed.
