The Rat DMD (Dystrophin) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of dystrophin levels in rat samples. This kit is suitable for use with serum, plasma, and cell culture supernatants, providing reliable and reproducible results for a variety of research applications.Dystrophin is a structural protein that plays a critical role in muscle function and stability. Mutations in the dystrophin gene are associated with Duchenne muscular dystrophy, a severe and progressive muscle-wasting disease. Monitoring dystrophin levels in animal models is essential for studying disease progression and evaluating potential therapeutic interventions.
Whether investigating muscle physiology, studying neuromuscular disorders, or developing novel treatments for muscular dystrophy, the Rat DMD CLIA Kit offers researchers a valuable tool for measuring dystrophin expression in rat models. Its high sensitivity and specificity make it an indispensable resource for advancing our understanding of dystrophin biology and exploring new avenues for therapeutic development.
Product Name:
Rat DMD (Dystrophin) CLIA Kit
SKU:
RTES00189
Target:
Rat DMD (Dystrophin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat DMD. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat DMD and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat DMD, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat DMD. You can calculate the concentration of Rat DMD in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-103
103-119
90-107
Average (%)
96
109
97
1:4
Range (%)
98-111
95-108
90-101
Average (%)
104
101
95
1:8
Range (%)
86-100
85-98
101-117
Average (%)
92
90
107
1:16
Range (%)
89-100
98-115
98-114
Average (%)
94
106
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-107
101
EDTA plasma (n=5)
92-103
98
Cell culture media (n=5)
97-110
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.55
1.05
3.86
0.6
0.98
3.52
Standard deviation
0.07
0.11
0.29
0.05
0.07
0.3
C V (%)
12.73
10.48
7.51
8.33
7.14
8.52
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat DMD concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat DMD in samples. No significant cross-reactivity or interference between Rat DMD and analogues was observed.
