The Rat DRD2 (Dopamine Receptor D2) CLIA Kit is specifically designed for the accurate detection of DRD2 levels in rat samples, including serum, plasma, and tissue lysates. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for research purposes.DRD2 is a vital dopamine receptor that plays a key role in regulating neurotransmission and neuronal function. Dysregulation of DRD2 has been implicated in various neurological and psychiatric disorders, including Parkinson's disease, schizophrenia, and addiction.
Therefore, measuring DRD2 levels can provide valuable insights into the pathophysiology of these conditions and aid in the development of novel therapeutic strategies.Overall, the Rat DRD2 CLIA Kit is a valuable tool for researchers studying the role of dopamine receptors in health and disease, offering precise and accurate measurements for detailed experimental analysis.
Product Name:
Rat DRD2 (Dopamine Receptor D2) CLIA Kit
SKU:
RTES00184
Target:
Rat DRD2 (Dopamine Receptor D2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 mIU/mL
Detection range:
0.31-20 mIU/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat DRD2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat DRD2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat DRD2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat DRD2. You can calculate the concentration of Rat DRD2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-113
94-110
84-100
Average (%)
106
100
91
1:4
Range (%)
98-113
103-115
85-100
Average (%)
105
109
91
1:8
Range (%)
100-113
90-106
101-117
Average (%)
107
97
109
1:16
Range (%)
94-108
99-116
94-105
Average (%)
101
106
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
93
EDTA plasma (n=5)
84-98
90
Cell culture media (n=5)
96-109
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.05
2.58
7.97
0.98
2.71
7.81
Standard deviation
0.1
0.28
0.55
0.12
0.2
0.79
C V (%)
9.52
10.85
6.9
12.24
7.38
10.12
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat DRD2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat DRD2 in samples. No significant cross-reactivity or interference between Rat DRD2 and analogues was observed.
