The Rat DRD3 (Dopamine Receptor D3) CLIA Kit is a highly sensitive and specific assay designed for the precise measurement of dopamine receptor D3 levels in rat serum, plasma, and tissue homogenates. This kit provides reliable and reproducible results, making it suitable for a variety of research applications.The dopamine receptor D3 is a key player in regulating dopamine signaling in the brain, influencing various physiological processes such as reward, motivation, and motor function.
Dysregulation of this receptor has been implicated in disorders like schizophrenia, addiction, and Parkinson's disease, making it an important target for therapeutic development.By accurately measuring dopamine receptor D3 levels, researchers can gain valuable insights into the role of this receptor in health and disease, paving the way for the development of novel treatments and interventions.
Product Name:
Rat DRD3 (Dopamine Receptor D3) CLIA Kit
SKU:
RTES00185
Target:
Rat DRD3 (Dopamine Receptor D3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat DRD3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat DRD3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat DRD3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat DRD3. You can calculate the concentration of Rat DRD3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-113
86-100
99-115
Average (%)
107
92
105
1:4
Range (%)
89-102
96-110
88-104
Average (%)
96
102
96
1:8
Range (%)
90-102
90-105
88-100
Average (%)
95
96
93
1:16
Range (%)
87-100
93-104
95-110
Average (%)
93
99
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-112
103
EDTA plasma (n=5)
88-101
95
Cell culture media (n=5)
99-115
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
219.85
510.66
1716.98
216.22
557.03
1623.01
Standard deviation
23.28
56.27
174.79
24.71
58.88
148.99
C V (%)
10.59
11.02
10.18
11.43
10.57
9.18
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat DRD3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat DRD3 in samples. No significant cross-reactivity or interference between Rat DRD3 and analogues was observed.
