The Rat DRD4 (Dopamine Receptor D4) CLIA Kit is a highly accurate and reliable tool for the detection of DRD4 levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise measurements for various research applications.The DRD4 receptor is a key component in the dopamine signaling pathway, playing a crucial role in neurological processes such as reward, addiction, and motor control.
Dysregulation of DRD4 has been linked to several psychiatric disorders, making it a valuable biomarker for studying these conditions and developing targeted therapies.With its advanced technology and easy-to-use format, the Rat DRD4 CLIA Kit is an essential tool for researchers seeking to understand the role of DRD4 in physiological and pathological processes in rats.
Product Name:
Rat DRD4 (Dopamine Receptor D4) CLIA Kit
SKU:
RTES00186
Target:
Rat DRD4 (Dopamine Receptor D4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat DRD4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat DRD4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat DRD4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat DRD4. You can calculate the concentration of Rat DRD4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-103
96-106
91-107
Average (%)
97
101
98
1:4
Range (%)
87-99
102-115
95-110
Average (%)
92
109
100
1:8
Range (%)
95-107
89-104
103-116
Average (%)
101
96
109
1:16
Range (%)
86-99
85-96
93-105
Average (%)
91
91
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-114
107
EDTA plasma (n=5)
94-108
101
Cell culture media (n=5)
87-100
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
94.73
214.58
774.08
98.17
195.37
839.44
Standard deviation
7.93
17.4
77.49
12.56
19.71
88.56
C V (%)
8.37
8.11
10.01
12.79
10.09
10.55
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat DRD4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat DRD4 in samples. No significant cross-reactivity or interference between Rat DRD4 and analogues was observed.
