Rat ECF/CCL11 (Eosinophil Chemotactic Factor) CLIA Kit
The Rat ECF (CCL11) Eosinophil Chemotactic Factor CLIA Kit (RTES00201) is a comprehensive assay designed for the precise measurement of ECF levels in rat serum, plasma, and tissue samples. This CLIA kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.ECF, also known as CCL11, is a key chemokine involved in the recruitment and activation of eosinophils, playing a crucial role in inflammatory responses and allergic reactions.
Elevated ECF levels have been linked to asthma, allergic diseases, and other inflammatory conditions, making this kit an invaluable tool for studying these processes and developing new therapeutic approaches.Overall, the Rat ECF (CCL11) Eosinophil Chemotactic Factor CLIA Kit (RTES00201) is a reliable and efficient solution for researchers seeking to investigate the role of ECF in health and disease in rat models.
Product Name:
Rat ECF/CCL11 (Eosinophil Chemotactic Factor) CLIA Kit
SKU:
RTES00201
Target:
Rat ECF/CCL11 (Eosinophil Chemotactic Factor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat ECF/CCL11. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ECF/CCL11 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ECF/CCL11, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat ECF/CCL11. You can calculate the concentration of Rat ECF/CCL11 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-112
102-117
87-100
Average (%)
104
110
92
1:4
Range (%)
88-99
93-109
92-104
Average (%)
93
100
98
1:8
Range (%)
97-110
86-101
100-114
Average (%)
103
93
108
1:16
Range (%)
95-107
94-106
99-114
Average (%)
101
101
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-110
105
EDTA plasma (n=5)
95-113
103
Cell culture media (n=5)
99-114
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
38.33
127.95
338.88
36.33
116.48
342.6
Standard deviation
3.38
10.1
32.3
4.12
10.15
22.92
C V (%)
8.82
7.89
9.53
11.34
8.71
6.69
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat ECF/CCL11 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat ECF/CCL11 in samples. No significant cross-reactivity or interference between Rat ECF/CCL11 and analogues was observed.
