Rat EG-VEGF (Endocrine Gland Vascular Endothelial Growth Factor) CLIA Kit
The Rat EG-VEGF (Endocrine Gland Vascular Endothelial Growth Factor) CLIA Kit is a highly sensitive and specific assay designed for the quantitative detection of EG-VEGF levels in rat samples including serum, plasma, and tissue homogenates. This kit provides reliable and reproducible results, making it a valuable tool for studying the role of EG-VEGF in endocrine gland function and vascular endothelial growth.EG-VEGF is a critical factor in regulating vascular development and angiogenesis specifically in endocrine glands.
Dysregulation of EG-VEGF has been linked to various endocrine disorders and diseases, making it a key biomarker for understanding these conditions and developing potential therapeutic interventions.Overall, the Rat EG-VEGF CLIA Kit is an essential research tool for investigating the role of EG-VEGF in endocrine gland function and vascular endothelial growth in rats.
Product Name:
Rat EG-VEGF (Endocrine Gland Vascular Endothelial Growth Factor) CLIA Kit
SKU:
RTES00195
Target:
Rat EG-VEGF (Endocrine Gland Vascular Endothelial Growth Factor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat EG-VEGF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat EG-VEGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat EG-VEGF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat EG-VEGF. You can calculate the concentration of Rat EG-VEGF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-119
100-116
86-99
Average (%)
108
106
92
1:4
Range (%)
90-100
100-115
102-117
Average (%)
95
106
108
1:8
Range (%)
101-116
98-114
101-119
Average (%)
107
106
109
1:16
Range (%)
95-111
99-114
92-106
Average (%)
102
107
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
99
EDTA plasma (n=5)
95-107
101
Cell culture media (n=5)
87-102
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
37.58
87.42
290.85
40.15
88.15
274.53
Standard deviation
3.57
10.1
29.06
5.11
10.45
22.4
C V (%)
9.5
11.55
9.99
12.73
11.85
8.16
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat EG-VEGF concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat EG-VEGF in samples. No significant cross-reactivity or interference between Rat EG-VEGF and analogues was observed.
