The Rat EGF (Epidermal Growth Factor) CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of EGF levels in rat serum, plasma, and tissue lysates. This kit provides reliable and reproducible results, making it ideal for a variety of research applications. EGF is a key growth factor that plays a crucial role in cell proliferation, differentiation, and tissue repair. It is involved in various physiological processes, including wound healing, development, and differentiation. Dysregulation of EGF signaling is associated with a range of diseases, including cancer, inflammatory disorders, and metabolic disorders, highlighting the importance of studying EGF levels in experimental models.
The Rat EGF CLIA Kit offers a simple and efficient way to measure EGF levels in rat samples, enabling researchers to gain valuable insights into the role of EGF in health and disease. This kit is a valuable tool for studying EGF signaling pathways, identifying potential therapeutic targets, and advancing our understanding of cellular growth and repair mechanisms.
Product Name:
Rat EGF (Epidermal Growth Factor) CLIA Kit
SKU:
RTES00202
Target:
Rat EGF (Epidermal Growth Factor)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat EGF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat EGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat EGF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat EGF. You can calculate the concentration of Rat EGF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-112
98-112
98-115
Average (%)
106
105
105
1:4
Range (%)
86-96
102-116
101-120
Average (%)
91
109
109
1:8
Range (%)
90-105
95-107
91-104
Average (%)
98
102
97
1:16
Range (%)
100-112
97-109
100-113
Average (%)
106
103
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-110
102
EDTA plasma (n=5)
95-111
103
Cell culture media (n=5)
91-105
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.73
66.18
173.33
22.29
68.29
169.81
Standard deviation
2.18
5.92
19.19
2.17
5.73
17.93
C V (%)
8.82
8.95
11.07
9.74
8.39
10.56
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat EGF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat EGF in samples. No significant cross-reactivity or interference between Rat EGF and analogues was observed.
