Rat EGR1 (Early Growth Response Protein 1) CLIA Kit
The Rat EGR1 (Early Growth Response Protein 1) CLIA Kit is a highly sensitive and specific assay designed for the quantitative detection of EGR1 levels in rat samples such as serum, plasma, and tissue lysates. This kit provides accurate and reproducible results, making it ideal for a variety of research applications.EGR1 is a key transcription factor that regulates gene expression in response to various stimuli, playing important roles in cell growth, differentiation, and response to stress.
Dysregulation of EGR1 has been linked to several diseases including cancer, cardiovascular disorders, and neurological conditions, highlighting its potential as a biomarker for understanding disease mechanisms and identifying therapeutic targets.Overall, the Rat EGR1 CLIA Kit offers researchers a reliable tool for studying the role of EGR1 in biological processes and disease pathogenesis, paving the way for advancements in basic science and clinical research.
Product Name:
Rat EGR1 (Early Growth Response Protein 1) CLIA Kit
SKU:
RTES00191
Target:
Rat EGR1 (Early Growth Response Protein 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
1.88 pg/mL
Detection range:
3.13-200 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat EGR1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat EGR1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat EGR1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat EGR1. You can calculate the concentration of Rat EGR1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-111
92-106
88-102
Average (%)
105
98
95
1:4
Range (%)
91-106
99-115
99-112
Average (%)
97
106
105
1:8
Range (%)
89-104
99-113
98-114
Average (%)
96
105
104
1:16
Range (%)
95-110
93-104
94-109
Average (%)
101
99
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-104
98
EDTA plasma (n=5)
98-111
103
Cell culture media (n=5)
92-105
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
10.66
27.53
70.52
11.32
28.35
74.4
Standard deviation
1.28
3.1
5.12
1.29
3.19
5.93
C V (%)
12.01
11.26
7.26
11.4
11.25
7.97
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat EGR1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat EGR1 in samples. No significant cross-reactivity or interference between Rat EGR1 and analogues was observed.
