Rat EGR2 (Early Growth Response Protein 2) CLIA Kit
The Rat EGR2 (Early Growth Response Protein 2) CLIA Kit is specifically designed for the accurate detection of EGR2 levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring consistent and trustworthy results for a variety of research applications.EGR2 is a vital transcription factor that regulates gene expression and plays a crucial role in cellular growth and differentiation. It is involved in various biological processes, including myelination, immune response, and nerve development.
The dysregulation of EGR2 has been linked to neurological disorders, autoimmune diseases, and cancer, highlighting its importance as a biomarker for studying these conditions and potential therapeutic interventions.Overall, the Rat EGR2 CLIA Kit provides researchers with a reliable tool for investigating the role of EGR2 in health and disease, offering valuable insights into its function and potential clinical applications.
Product Name:
Rat EGR2 (Early Growth Response Protein 2) CLIA Kit
SKU:
RTES00192
Target:
Rat EGR2 (Early Growth Response Protein 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat EGR2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat EGR2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat EGR2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat EGR2. You can calculate the concentration of Rat EGR2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-100
85-97
91-108
Average (%)
95
91
98
1:4
Range (%)
85-99
101-118
99-116
Average (%)
92
109
107
1:8
Range (%)
101-117
89-103
83-97
Average (%)
109
95
90
1:16
Range (%)
94-109
92-103
93-106
Average (%)
101
97
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-104
95
EDTA plasma (n=5)
97-113
103
Cell culture media (n=5)
100-112
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
197.35
449.3
1828.86
203.89
442.92
1988.48
Standard deviation
20.56
45.96
148.32
24.92
46.68
143.37
C V (%)
10.42
10.23
8.11
12.22
10.54
7.21
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat EGR2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat EGR2 in samples. No significant cross-reactivity or interference between Rat EGR2 and analogues was observed.
