The Rat EP2 (Prostaglandin E Receptor 2) CLIA Kit is a powerful tool designed for the accurate measurement of EP2 levels in rat samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.EP2, a member of the prostaglandin E receptor family, plays a crucial role in mediating the effects of prostaglandin E2, a key inflammatory mediator.
Understanding the expression levels of EP2 can provide valuable insights into the inflammatory response and may offer potential therapeutic targets for diseases such as inflammation, pain, and cancer.With its easy-to-use format and quick assay time, the Rat EP2 CLIA Kit is an essential tool for researchers studying the role of EP2 in various physiological and pathological processes. Upgrade your research with this innovative kit from Assay Genie.
Product Name:
Rat EP2 (Prostaglandin E Receptor 2) CLIA Kit
SKU:
RTES00475
Target:
Rat EP2 (Prostaglandin E Receptor 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat EP2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat EP2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat EP2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat EP2. You can calculate the concentration of Rat EP2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-103
101-115
87-98
Average (%)
94
107
93
1:4
Range (%)
103-119
95-109
90-105
Average (%)
110
103
96
1:8
Range (%)
91-107
95-111
104-116
Average (%)
99
103
110
1:16
Range (%)
96-113
94-108
98-112
Average (%)
104
100
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-106
98
EDTA plasma (n=5)
87-97
92
Cell culture media (n=5)
94-106
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
40.19
82.78
345.36
41.83
80.65
341.55
Standard deviation
4.03
6.75
37.64
3.36
6.35
28.28
C V (%)
10.03
8.15
10.9
8.03
7.87
8.28
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat EP2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat EP2 in samples. No significant cross-reactivity or interference between Rat EP2 and analogues was observed.
