Rat ERN1 (Endoplasmic Reticulum To Nucleus Signalling 1) CLIA Kit
The Rat ERN1 (Endoplasmic Reticulum to Nucleus Signalling 1) CLIA Kit is a highly reliable and sensitive assay designed for the precise detection of ERN1 levels in rat samples. This kit offers exceptional specificity and accuracy, ensuring consistent and trustworthy results for a variety of research applications.ERN1 is a critical protein involved in endoplasmic reticulum stress response and nucleus signaling pathways, playing a key role in cellular homeostasis and stress adaptation. Dysregulation of ERN1 has been linked to various diseases, including cancer, diabetes, and neurodegenerative disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.
With its advanced technology and superior performance, the Rat ERN1 CLIA Kit is an indispensable tool for researchers investigating the intricate mechanisms of ER stress and nucleus signaling in rat models. Get accurate and insightful results with this innovative assay kit from Assay Genie.
Product Name:
Rat ERN1 (Endoplasmic Reticulum To Nucleus Signalling 1) CLIA Kit
SKU:
RTES00197
Target:
Rat ERN1 (Endoplasmic Reticulum To Nucleus Signalling 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat ERN1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ERN1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ERN1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat ERN1. You can calculate the concentration of Rat ERN1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-103
89-103
101-118
Average (%)
97
96
108
1:4
Range (%)
99-118
89-105
99-115
Average (%)
107
96
106
1:8
Range (%)
97-110
92-102
102-118
Average (%)
102
97
109
1:16
Range (%)
90-104
89-101
96-109
Average (%)
97
95
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
94
EDTA plasma (n=5)
91-106
98
Cell culture media (n=5)
85-100
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.65
51.27
170.62
22.93
47.28
170.01
Standard deviation
2.13
6.12
18.26
1.83
4.86
18.26
C V (%)
8.64
11.94
10.7
7.98
10.28
10.74
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat ERN1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat ERN1 in samples. No significant cross-reactivity or interference between Rat ERN1 and analogues was observed.
