Description
Rat ES (Endostatin) ELISA Kit
The Rat ES (Endostatin) ELISA Kit is a specialized assay designed for the quantitative detection of endostatin levels in rat biological samples. Endostatin is a fragment of collagen XVIII that inhibits endothelial cell proliferation, migration, and angiogenesis. As a potent anti-angiogenic factor, endostatin plays a crucial role in regulating blood vessel formation and tumor growth by disrupting the tumor vasculature. Quantifying endostatin levels can provide valuable insights into angiogenic processes, tumor progression, and potential therapeutic targets. Changes in endostatin levels have been associated with various conditions, including cancer, cardiovascular diseases, and inflammatory disorders.
Therefore, accurate measurement of endostatin is essential for understanding its impact on angiogenesis and disease development. The Rat ES ELISA Kit offered by Assay Genie is designed to deliver precise and reliable results. With exceptional sensitivity and specificity, this kit ensures accurate quantification of endostatin levels in biological samples. Manufactured under stringent quality control standards, the kit provides robust performance and ease of use, making it an excellent choice for research focused on angiogenesis, tumor biology, and vascular disorders.
| Product Name: | Rat ES (Endostatin) ELISA Kit |
| Product Code: | AEES00391 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Rat |
| Detection Range: | 0.16-10 ng/mL |
| Sensitivity: | 0.10 ng/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100?L |
| Specificity: | This kit recognizes Rat ES in samples. No significant cross-reactivity or interference between Rat ES and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Rat ES concentrations in Serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ES. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ES and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ES, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ES. You can calculate the concentration of Rat ES in the samples by comparing the OD of the samples to the standard curve.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat ES were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat ES were tested on 3 different plates, 20 replicates in each plate.
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.49 | 1.03 | 3.91 | 0.47 | 1.00 | 4.08 |
| Standard deviation | 0.03 | 0.06 | 0.16 | 0.03 | 0.04 | 0.20 |
| CV (%) | 6.12 | 5.83 | 4.09 | 6.38 | 4.00 | 4.90 |
Recovery
The recovery of Rat ES spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 95-108 | 101 |
| EDTA plasma (n=8) | 87-99 | 93 |
| Cell culture media (n=8) | 86-97 | 91 |
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
|
| 1. | Add 100?L standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100?L Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100?L HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90?L Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50?L Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
