The Rat F2 (Coagulation Factor 2) CLIA Kit is specifically designed for the quantitative detection of coagulation factor 2 levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.Coagulation factor 2, also known as prothrombin, is a key protein in the blood coagulation pathway, playing a critical role in clot formation and wound healing.
Dysregulation of coagulation factor 2 levels has been associated with various blood clotting disorders and cardiovascular diseases, making it a valuable biomarker for studying these conditions and developing potential treatments.Overall, the Rat F2 CLIA Kit provides researchers with a valuable tool for investigating the role of coagulation factor 2 in health and disease, with the potential to advance our understanding and treatment of related conditions.
Product Name:
Rat F2 (Coagulation Factor 2) CLIA Kit
SKU:
RTES00128
Target:
Rat F2 (Coagulation Factor 2)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat F2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat F2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat F2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat F2. You can calculate the concentration of Rat F2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-117
90-105
103-120
Average (%)
107
96
110
1:4
Range (%)
91-105
93-105
97-110
Average (%)
97
98
104
1:8
Range (%)
95-108
89-103
100-117
Average (%)
103
97
108
1:16
Range (%)
99-114
96-114
97-107
Average (%)
106
104
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-110
100
EDTA plasma (n=5)
93-108
99
Cell culture media (n=5)
97-112
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
103.17
317.78
775.85
94.18
299.37
812.57
Standard deviation
9.11
28.82
80.92
9.95
21.17
68.42
C V (%)
8.83
9.07
10.43
10.56
7.07
8.42
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat F2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat F2 in samples. No significant cross-reactivity or interference between Rat F2 and analogues was observed.
