Based on the information in the URL provided, the Rat F3 (Coagulation Factor 3) CLIA Kit offered by Assay Genie is a reliable and accurate tool for measuring levels of coagulation factor 3 in rat samples. This kit is specifically designed for use with rat serum, plasma, and cell culture supernatants, providing researchers with valuable insights into the coagulation pathways in rats.Coagulation factor 3, also known as tissue factor, is a key player in the blood coagulation cascade, initiating the formation of blood clots in response to injury.
Abnormalities in the levels of coagulation factor 3 can lead to various hemostasis disorders and thrombotic events, making it an important biomarker for studying coagulation-related diseases.With its high sensitivity and specificity, the Rat F3 CLIA Kit from Assay Genie ensures precise and reproducible results, making it a valuable asset for researchers studying coagulation disorders, cardiovascular diseases, and other conditions related to blood clot formation in rats.
Product Name:
Rat F3 (Coagulation Factor 3) CLIA Kit
SKU:
RTES00129
Target:
Rat F3 (Coagulation Factor 3)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat F3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat F3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat F3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat F3. You can calculate the concentration of Rat F3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-101
93-107
101-117
Average (%)
94
100
108
1:4
Range (%)
85-98
90-102
100-117
Average (%)
92
97
108
1:8
Range (%)
99-116
91-104
94-110
Average (%)
107
99
101
1:16
Range (%)
87-101
101-120
100-117
Average (%)
94
109
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-110
105
EDTA plasma (n=5)
88-103
95
Cell culture media (n=5)
86-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
41.64
103.42
358.66
41.79
102.56
363.45
Standard deviation
3.83
12.41
34.04
3.63
12.19
37.11
C V (%)
9.2
12.0
9.49
8.69
11.89
10.21
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat F3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat F3 in samples. No significant cross-reactivity or interference between Rat F3 and analogues was observed.
