The Rat Fas (CD95) Factor Related Apoptosis CLIA Kit is specifically designed for the precise measurement of Fas (CD95) levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.Fas (CD95) is a key protein involved in apoptosis, the process of programmed cell death. Dysregulation of Fas signaling has been linked to various pathological conditions, including cancer, autoimmune disorders, and neurodegenerative diseases.
By accurately quantifying Fas levels, researchers can gain valuable insights into the mechanisms underlying these conditions and potential therapeutic strategies.Overall, the Rat Fas (CD95) Factor Related Apoptosis CLIA Kit is a valuable tool for research scientists studying apoptosis, cell death mechanisms, and related diseases in rat models. Its reliable performance and user-friendly protocol make it an essential addition to any laboratory investigating Fas signaling pathways.
Product Name:
Rat FAS/CD95 (Factor Related Apoptosis) CLIA Kit
SKU:
RTES00206
Target:
Rat FAS/CD95 (Factor Related Apoptosis)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat FAS/CD95. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat FAS/CD95 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat FAS/CD95, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat FAS/CD95. You can calculate the concentration of Rat FAS/CD95 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-108
100-116
100-114
Average (%)
101
107
107
1:4
Range (%)
92-108
88-100
92-105
Average (%)
100
95
99
1:8
Range (%)
103-119
87-100
97-112
Average (%)
109
93
103
1:16
Range (%)
92-107
94-111
99-116
Average (%)
99
101
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
97
EDTA plasma (n=5)
94-108
99
Cell culture media (n=5)
102-118
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
43.08
72.78
367.24
42.04
75.8
373.36
Standard deviation
3.66
7.45
26.26
5.04
5.73
25.84
C V (%)
8.5
10.24
7.15
11.99
7.56
6.92
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat FAS/CD95 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat FAS/CD95 in samples. No significant cross-reactivity or interference between Rat FAS/CD95 and analogues was observed.
