Rat FASL (Factor Related Apoptosis Ligand) CLIA Kit
The Rat Fasl (Factor Related Apoptosis Ligand) CLIA Kit is a cutting-edge tool for detecting levels of Fasl in rat serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and reproducible results, making it an invaluable asset for various research applications.Fasl is a key protein involved in apoptosis, playing a vital role in regulating cell death processes.
Dysregulation of Fasl has been implicated in various diseases, including cancer and autoimmune disorders, highlighting its importance as a potential therapeutic target. This CLIA kit provides researchers with the means to accurately measure Fasl levels, enabling deeper insights into its biological functions and potential clinical applications.
Product Name:
Rat FASL (Factor Related Apoptosis Ligand) CLIA Kit
SKU:
RTES00635
Target:
Rat FASL (Factor Related Apoptosis Ligand)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat FASL. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat FASL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat FASL, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat FASL. You can calculate the concentration of Rat FASL in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-115
89-101
87-99
Average (%)
109
95
93
1:4
Range (%)
96-110
86-100
99-112
Average (%)
102
92
105
1:8
Range (%)
92-104
92-104
96-109
Average (%)
98
99
104
1:16
Range (%)
97-112
95-111
88-98
Average (%)
104
101
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
96
EDTA plasma (n=5)
92-106
98
Cell culture media (n=5)
101-114
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
41.25
126.41
332.24
41.34
127.2
311.22
Standard deviation
5.08
12.01
27.21
3.83
10.2
29.57
C V (%)
12.32
9.5
8.19
9.26
8.02
9.5
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat FASL concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat FASL in samples. No significant cross-reactivity or interference between Rat FASL and analogues was observed.
