The Rat FDP (Fibrinogen Degradation Product) CLIA Kit is a powerful tool for detecting levels of fibrinogen degradation products in rat samples. With high sensitivity and specificity, this kit provides accurate and reliable results, making it ideal for various research applications.Fibrinogen degradation products are key indicators of fibrinolysis, the process of breaking down fibrin blood clots. Monitoring these products can provide valuable insights into clotting disorders, cardiovascular diseases, and inflammatory conditions.
By using the Rat FDP CLIA Kit, researchers can gain a deeper understanding of fibrinolytic processes in rats and explore potential therapeutic targets for various diseases. This kit is essential for studying clotting mechanisms and developing novel treatment strategies.
Product Name:
Rat FDP (Fibrinogen Degradation Product) CLIA Kit
SKU:
RTES00209
Target:
Rat FDP (Fibrinogen Degradation Product)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat FDP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat FDP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat FDP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat FDP. You can calculate the concentration of Rat FDP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-112
98-114
90-104
Average (%)
103
105
97
1:4
Range (%)
88-100
87-101
101-115
Average (%)
93
93
108
1:8
Range (%)
92-106
86-98
89-104
Average (%)
97
93
95
1:16
Range (%)
93-104
98-114
98-113
Average (%)
99
104
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-111
102
EDTA plasma (n=5)
84-96
90
Cell culture media (n=5)
94-109
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.53
55.28
225.79
28.49
55.4
225.36
Standard deviation
2.53
4.51
23.82
2.77
5.88
14.51
C V (%)
9.54
8.16
10.55
9.72
10.61
6.44
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat FDP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat FDP in samples. No significant cross-reactivity or interference between Rat FDP and analogues was observed.
