Description
Rat FDP (Fibrinogen Degradation Product) ELISA Kit
The Rat FDP (Fibrinogen Degradation Product) ELISA Kit is a high-quality assay designed for the quantitative detection of Fibrinogen Degradation Product levels in various rat biological samples. Fibrinogen Degradation Products are key markers of fibrinolysis, the process of breaking down fibrin clots in response to an injury. Monitoring FDP levels is crucial in understanding the status of fibrinolysis and coagulation cascade activity in the body. Accurate quantification of Fibrinogen Degradation Products using this ELISA kit enables researchers to assess the activation of fibrinolysis, which is essential for various physiological and pathological processes. Changes in fibrinolysis are associated with conditions like disseminated intravascular coagulation (DIC), thrombosis, and liver disease, making FDP a valuable biomarker for these disorders.
The Rat FDP ELISA Kit from Assay Genie offers exceptional sensitivity and specificity, ensuring precise and reproducible results. Manufactured under strict quality control standards, this kit provides reliable performance and delivers accurate data on Fibrinogen Degradation Product levels in biological samples. It serves as a valuable tool for researchers investigating coagulation disorders and studying the intricate balance between coagulation and fibrinolysis in various physiological and disease states.
| Product Name: | Rat FDP (Fibrinogen Degradation Product) ELISA Kit |
| Product Code: | AEES00397 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Rat |
| Detection Range: | 23.44-1500 pg/mL |
| Sensitivity: | 14.06 pg/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100?L |
| Specificity: | This kit recognizes Rat FDP in samples. No significant cross-reactivity or interference between Rat FDP and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Rat FDP concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat FDP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat FDP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat FDP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat FDP. You can calculate the concentration of Rat FDP in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
|
| 1. | Add 100?L standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100?L Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100?L HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90?L Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50?L Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
