The Rat FGF6 (Fibroblast Growth Factor 6) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of FGF6 levels in rat samples including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.FGF6 is a growth factor that plays a crucial role in cell growth, differentiation, and tissue repair. It is involved in various biological processes such as wound healing, muscle regeneration, and development of the nervous system.
Abnormal levels of FGF6 have been associated with diseases such as cancer, fibrosis, and cardiovascular disorders, highlighting its importance as a potential biomarker for disease research and drug development.With its high sensitivity and specificity, the Rat FGF6 CLIA Kit offers researchers a valuable tool for studying the role of FGF6 in health and disease, ultimately leading to a better understanding of its therapeutic potential.
Product Name:
Rat FGF6 (Fibroblast Growth Factor 6) CLIA Kit
SKU:
RTES00054
Target:
Rat FGF6 (Fibroblast Growth Factor 6)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat FGF6. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat FGF6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat FGF6, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat FGF6. You can calculate the concentration of Rat FGF6 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-106
94-106
104-120
Average (%)
97
100
110
1:4
Range (%)
91-104
85-100
86-100
Average (%)
97
92
91
1:8
Range (%)
101-116
99-115
103-115
Average (%)
107
106
109
1:16
Range (%)
92-103
91-106
95-113
Average (%)
98
98
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
96
EDTA plasma (n=5)
102-114
107
Cell culture media (n=5)
89-103
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.18
79.48
227.89
26.53
81.92
222.23
Standard deviation
2.84
8.47
13.76
2.82
6.89
21.62
C V (%)
11.28
10.66
6.04
10.63
8.41
9.73
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat FGF6 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat FGF6 in samples. No significant cross-reactivity or interference between Rat FGF6 and analogues was observed.
