The Rat FGF9 (Fibroblast Growth Factor 9) CLIA Kit is specifically designed for the accurate measurement of FGF9 levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.FGF9 is a key protein involved in cell growth and differentiation, particularly in the development of various tissues and organs. It plays a crucial role in processes such as embryonic development, tissue repair, and wound healing.
Aberrant FGF9 expression has been linked to various diseases, including cancer and developmental disorders, making it an important target for research and potential therapeutic interventions.With its advanced technology and reliable performance, the Rat FGF9 CLIA Kit is a valuable tool for researchers studying the biological functions of FGF9 and its implications in disease pathogenesis. Get accurate and precise results with this innovative kit from Assay Genie.
Product Name:
Rat FGF9 (Fibroblast Growth Factor 9) CLIA Kit
SKU:
RTES00055
Target:
Rat FGF9 (Fibroblast Growth Factor 9)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat FGF9. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat FGF9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat FGF9, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat FGF9. You can calculate the concentration of Rat FGF9 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-102
95-108
98-115
Average (%)
95
100
106
1:4
Range (%)
99-112
87-102
98-110
Average (%)
105
93
103
1:8
Range (%)
103-118
91-103
89-104
Average (%)
109
96
95
1:16
Range (%)
89-101
87-100
89-103
Average (%)
96
94
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-117
107
EDTA plasma (n=5)
91-104
98
Cell culture media (n=5)
98-109
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
46.79
146.4
392.57
48.44
155.64
421.77
Standard deviation
4.66
11.11
35.88
6.29
11.27
43.02
C V (%)
9.96
7.59
9.14
12.99
7.24
10.2
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat FGF9 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat FGF9 in samples. No significant cross-reactivity or interference between Rat FGF9 and analogues was observed.
