The Rat Fibroblast Growth Factor-4 (FGF4) ELISA Kit is a powerful tool for the precise measurement of FGF4 levels in rat serum, plasma, and tissue lysates. With its exceptional sensitivity and specificity, this kit delivers accurate and dependable results, making it an indispensable resource for a variety of research activities.FGF4 is a key growth factor that plays a vital role in cell proliferation, differentiation, and tissue regeneration. Its dysregulation has been linked to various diseases, including cancer, developmental disorders, and wound healing complications.
By accurately measuring FGF4 levels, researchers can gain valuable insights into these conditions and explore potential therapeutic interventions. Overall, the Rat FGF4 ELISA Kit offers a reliable solution for studying the role of FGF4 in biological processes and disease pathogenesis, paving the way for new discoveries and advancements in scientific research.
Product Name:
Rat Fibroblast Growth Factor 4 (FGF4) ELISA Kit
Product Code:
RTDL00382
Size:
96 Assays
Target:
FGF4
Synonyms:
HBGF4, HST, HSTF1, KFGF, K-FGF, Heparin Secretory Transforming Protein 1, Kaposi Sarcoma Oncogene, Transforming Protein KS3, FGF-Related Oncogene
Detection Method:
Sandwich
Range:
15.625-1,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
