Rat Fibronectin Type III Domain Containing Protein 5 (FNDC5) ELISA Kit
The Rat FNDC5 (Fibronectin Type III Domain Containing Protein 5) ELISA Kit is a highly reliable and sensitive assay designed for the precise measurement of FNDC5 levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional accuracy and reproducibility, making it an excellent choice for a variety of research applications.FNDC5 is a critical protein involved in regulating metabolism and energy expenditure through the production of irisin, a hormone that can help combat obesity and related metabolic disorders.
Studies have shown that FNDC5 may also play a role in enhancing cognitive function and protecting against neurodegeneration, making it a valuable target for further investigation in the fields of obesity, neurology, and metabolic diseases. With its high sensitivity and specificity, the Rat FNDC5 ELISA Kit provides researchers with a valuable tool for studying the functions and potential therapeutic applications of FNDC5 in various disease models.
Product Name:
Rat Fibronectin Type III Domain Containing Protein 5 (FNDC5) ELISA Kit
Product Code:
RTDL00391
Size:
96 Assays
Target:
FNDC5
Synonyms:
FRCP2, Irisin, Fibronectin type III repeat-containing protein 2
Detection Method:
Sandwich
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
