The Rat Forkhead Box Protein O1 (FOXO1) ELISA Kit is specifically designed for the quantitative detection of FOXO1 levels in rat serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring accurate and reliable results for various research applications.FOXO1 is a transcription factor that plays a key role in regulating various cellular processes, including cell growth, differentiation, and survival. Dysregulation of FOXO1 has been associated with a range of diseases, such as cancer, diabetes, and cardiovascular disorders, making it a valuable target for research and potential therapeutic interventions.
By utilizing the Rat Forkhead Box Protein O1 (FOXO1) ELISA Kit, researchers can gain valuable insights into the role of FOXO1 in disease pathogenesis and explore its potential as a therapeutic target. This kit provides a convenient and efficient tool for studying FOXO1 in rat models, ultimately advancing our understanding of this important transcription factor.
Product Name:
Rat Forkhead Box Protein O1 (FOXO1) ELISA Kit
Product Code:
RTDL00393
Size:
96 Assays
Target:
FOXO1
Synonyms:
FOX-O1, FKH1, FKHR, FOXO1A, Forkhead Homolog In Rhabdomyosarcoma
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
