The Rat FST (Follistatin) CLIA Kit is a highly accurate and reliable tool for measuring follistatin levels in rat serum, plasma, and tissue extracts. This kit offers exceptional sensitivity and specificity, providing researchers with consistent and reproducible results for a variety of research applications.Follistatin is a key protein that regulates various physiological processes, including muscle growth, inflammation, and reproductive health.
Dysregulation of follistatin levels has been implicated in a variety of diseases, making it a valuable biomarker for studying and developing potential treatments for conditions such as muscle wasting disorders, inflammatory diseases, and infertility.Overall, the Rat FST (Follistatin) CLIA Kit is an essential tool for researchers looking to delve into the intricate mechanisms of follistatin biology and its implications for human health.
Product Name:
Rat FST (Follistatin) CLIA Kit
SKU:
RTES00212
Target:
Rat FST (Follistatin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat FST. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat FST and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat FST, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat FST. You can calculate the concentration of Rat FST in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-106
95-107
93-107
Average (%)
99
100
98
1:4
Range (%)
94-109
92-107
92-108
Average (%)
101
99
98
1:8
Range (%)
90-102
86-98
92-105
Average (%)
95
91
98
1:16
Range (%)
104-118
100-115
94-110
Average (%)
110
106
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-109
100
EDTA plasma (n=5)
93-109
101
Cell culture media (n=5)
87-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.45
185.99
901.72
91.8
199.14
892.43
Standard deviation
11.99
14.56
90.98
8.38
17.96
62.56
C V (%)
12.56
7.83
10.09
9.13
9.02
7.01
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat FST concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat FST in samples. No significant cross-reactivity or interference between Rat FST and analogues was observed.
