The Rat FSTL1 (Follistatin-like protein 1) CLIA Kit is a comprehensive solution for quantifying FSTL1 levels in rat serum, plasma, and tissue homogenates. This kit offers exceptional accuracy and precision, ensuring consistent and trustworthy results for a variety of research purposes.FSTL1 is a key regulator of various biological processes, including inflammation, tissue repair, and metabolism. Its dysregulation has been implicated in pathologies such as arthritis, cardiovascular diseases, and fibrosis, underscoring its significance as a potential therapeutic target.
With its superior performance and versatility, the Rat FSTL1 CLIA Kit is an indispensable tool for investigating the role of FSTL1 in health and disease, paving the way for new insights and treatment strategies in biomedical research.
Product Name:
Rat FSTL1 (Follistatin Like Protein 1) CLIA Kit
SKU:
RTES00467
Target:
Rat FSTL1 (Follistatin Like Protein 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat FSTL1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat FSTL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat FSTL1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat FSTL1. You can calculate the concentration of Rat FSTL1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-107
86-97
87-98
Average (%)
101
92
93
1:4
Range (%)
98-110
85-96
83-98
Average (%)
103
91
90
1:8
Range (%)
96-110
92-105
97-112
Average (%)
101
99
102
1:16
Range (%)
94-109
94-110
86-98
Average (%)
100
102
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-107
101
EDTA plasma (n=5)
85-101
92
Cell culture media (n=5)
101-117
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
52.44
110.0
425.75
57.28
102.1
394.88
Standard deviation
4.66
9.87
29.63
7.43
8.17
29.93
C V (%)
8.89
8.97
6.96
12.97
8.0
7.58
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat FSTL1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat FSTL1 in samples. No significant cross-reactivity or interference between Rat FSTL1 and analogues was observed.
