The Rat FX (Coagulation Factor X) CLIA Kit is specifically designed for the quantitative measurement of Coagulation Factor X levels in rat serum, plasma, and other biological samples. This kit offers high sensitivity and accuracy, providing researchers with reliable and reproducible results for a variety of research applications.Coagulation Factor X, also known as the Stuart-Prower factor, plays a critical role in the blood coagulation cascade by converting prothrombin to thrombin.
Dysregulation of Coagulation Factor X activity can lead to various bleeding disorders and thrombotic events, making it an important marker for studying coagulation-related diseases.By using the Rat FX CLIA Kit, researchers can gain valuable insights into the role of Coagulation Factor X in hemostasis and thrombosis, opening up new possibilities for understanding and treating coagulation disorders in rat models.
Product Name:
Rat FX (Coagulation Factor X) CLIA Kit
SKU:
RTES00130
Target:
Rat FX (Coagulation Factor X)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat F10. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat F10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat F10, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat F10. You can calculate the concentration of Rat F10 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-115
89-104
88-101
Average (%)
107
96
94
1:4
Range (%)
92-102
99-115
92-104
Average (%)
97
106
97
1:8
Range (%)
99-115
97-115
99-114
Average (%)
106
105
106
1:16
Range (%)
93-110
100-115
90-104
Average (%)
100
108
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
95
EDTA plasma (n=5)
89-103
95
Cell culture media (n=5)
87-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
442.82
1252.08
3842.61
413.7
1166.91
3760.41
Standard deviation
36.84
98.29
410.39
51.71
108.76
399.73
C V (%)
8.32
7.85
10.68
12.5
9.32
10.63
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat F10 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat F10 in samples. No significant cross-reactivity or interference between Rat F10 and analogues was observed.
