The Rat GAL (Galanin) CLIA Kit is a reliable and accurate tool for the detection of galanin levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Galanin is a vital neuropeptide involved in regulating various physiological functions, including pain modulation, anxiety, and feeding behavior. Its dysregulation has been linked to conditions such as Alzheimer's disease, epilepsy, and depression, making it a crucial biomarker for understanding these disorders and exploring potential therapeutic interventions.
Overall, the Rat GAL (Galanin) CLIA Kit is a valuable resource for researchers studying the role of galanin in neurobiology and disease pathogenesis, providing accurate and reliable data for advancing scientific knowledge and developing novel treatment strategies.
Product Name:
Rat GAL (Galanin) CLIA Kit
SKU:
RTES00221
Target:
Rat GAL (Galanin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GAL. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GAL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GAL, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GAL. You can calculate the concentration of Rat GAL in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-117
101-112
85-100
Average (%)
110
107
92
1:4
Range (%)
102-115
84-99
102-114
Average (%)
108
91
108
1:8
Range (%)
91-102
89-101
102-118
Average (%)
96
95
107
1:16
Range (%)
88-103
89-106
90-104
Average (%)
95
97
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
99
EDTA plasma (n=5)
96-109
103
Cell culture media (n=5)
100-117
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.16
73.25
178.01
27.25
74.66
165.66
Standard deviation
2.72
7.22
10.88
2.76
5.86
13.3
C V (%)
10.01
9.86
6.11
10.13
7.85
8.03
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GAL concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GAL in samples. No significant cross-reactivity or interference between Rat GAL and analogues was observed.
