Based on the information from the URL provided, the Rat Gal1 (Galectin-1) CLIA Kit offered by Assay Genie is a state-of-the-art assay kit designed for the accurate measurement of Galectin-1 levels in rat samples. Galectin-1 is a key protein involved in various physiological and pathological processes, including cell adhesion, inflammation, immune response, and cancer progression.This CLIA kit is specially optimized to provide high sensitivity and specificity, ensuring precise and reliable results in serum, plasma, and tissue samples.
With its user-friendly protocol and quick turnaround time, researchers can efficiently measure Galectin-1 levels in a wide range of experimental settings, making it an essential tool for studying the role of Galectin-1 in various disease conditions and therapeutic interventions.Overall, the Rat Gal1 CLIA Kit from Assay Genie is a valuable resource for researchers investigating the molecular mechanisms underlying Galectin-1 function and its potential as a diagnostic or therapeutic target in different disease contexts.
Product Name:
Rat GAL1 (Galectin 1) CLIA Kit
SKU:
RTES00222
Target:
Rat GAL1 (Galectin 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GAL1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GAL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GAL1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GAL1. You can calculate the concentration of Rat GAL1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-109
86-102
92-108
Average (%)
101
93
99
1:4
Range (%)
100-112
98-110
93-107
Average (%)
106
104
99
1:8
Range (%)
102-116
89-104
86-100
Average (%)
110
95
92
1:16
Range (%)
94-108
99-115
97-112
Average (%)
100
105
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-116
107
EDTA plasma (n=5)
88-104
95
Cell culture media (n=5)
101-116
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
419.98
835.63
3127.17
449.31
846.72
2945.74
Standard deviation
51.36
94.01
264.56
58.41
80.35
262.76
C V (%)
12.23
11.25
8.46
13.0
9.49
8.92
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GAL1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GAL1 in samples. No significant cross-reactivity or interference between Rat GAL1 and analogues was observed.
