The Rat GAL1 (Galectin 1) ELISA Kit is a cutting-edge assay meticulously engineered to quantitatively measure Galectin 1 levels in various rat biological samples. Galectin 1, a multifunctional carbohydrate-binding protein, participates in diverse cellular processes, including cell adhesion, migration, differentiation, and immune modulation.
Its role as a critical regulator in numerous physiological and pathological conditions highlights its significance as a molecular target for detailed investigations in research and clinical settings. This ELISA kit empowers scientists and clinicians to accurately assess Galectin 1 expression, facilitating a comprehensive understanding of its involvement in cell signaling cascades, inflammation, cancer progression, and immune responses. Precise quantification of Galectin 1 levels is essential for unraveling its intricate functions and deciphering its therapeutic potential in various disease contexts. Designed with precision and excellence, the Rat GAL1 ELISA Kit from Assay Genie ensures exceptional sensitivity and specificity, delivering reliable and reproducible results. Manufactured under rigorous quality standards, this kit offers robust performance and user-friendly protocols, making it a preferred choice for researchers and practitioners aiming to explore the intricate role of Galectin 1 in biological systems and disease pathophysiology.
Product Name:
Rat GAL1 (Galectin 1) ELISA Kit
SKU:
AEES00552
Target:
Rat GAL1 (Galectin 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GAL1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GAL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GAL1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat GAL1. You can calculate the concentration of Rat GAL1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-111
97-113
88-99
Average (%)
105
103
94
1:4
Range (%)
94-107
98-113
90-101
Average (%)
100
104
95
1:8
Range (%)
99-115
94-110
85-97
Average (%)
107
102
90
1:16
Range (%)
93-108
96-109
83-98
Average (%)
100
101
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-108
102
EDTA plasma (n=5)
94-108
102
Cell culture media (n=5)
87-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
105.05
315.71
871.55
107.76
302.48
865.57
Standard deviation
5.38
14.52
38.96
6.91
14.61
46.91
C V (%)
5.12
4.6
4.47
6.41
4.83
5.42
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat GAL1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GAL1 in samples. No significant cross-reactivity or interference between Rat GAL1 and analogues was observed.
