The Rat Gal2 (Galectin-2) CLIA Kit is a reliable and sensitive tool for the quantitative measurement of Galectin-2 levels in rat serum, plasma, and cell culture supernatants. This kit offers high specificity and accuracy, ensuring precise and reproducible results for various research applications.Galectin-2 is a key protein that plays a critical role in immune response modulation, cell adhesion, and apoptosis. It is involved in various physiological and pathological processes, including inflammation, autoimmune diseases, and cancer development.
By accurately measuring Galectin-2 levels, researchers can gain valuable insights into the mechanisms underlying these conditions and identify potential therapeutic targets.Overall, the Rat Gal2 CLIA Kit is an essential tool for studying Galectin-2 biology and its role in disease progression, providing researchers with valuable data to advance their research and potential therapeutic interventions.
Product Name:
Rat GAL2 (Galectin 2) CLIA Kit
SKU:
RTES00223
Target:
Rat GAL2 (Galectin 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GAL2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GAL2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GAL2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GAL2. You can calculate the concentration of Rat GAL2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-101
97-112
94-107
Average (%)
94
103
99
1:4
Range (%)
90-103
93-106
83-96
Average (%)
98
99
90
1:8
Range (%)
90-104
99-118
91-107
Average (%)
97
107
97
1:16
Range (%)
96-110
88-99
85-97
Average (%)
103
93
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-102
93
EDTA plasma (n=5)
98-114
105
Cell culture media (n=5)
94-105
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.81
48.16
183.28
26.24
46.99
183.46
Standard deviation
2.21
5.72
12.99
2.93
4.28
13.21
C V (%)
8.24
11.88
7.09
11.17
9.11
7.2
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GAL2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GAL2 in samples. No significant cross-reactivity or interference between Rat GAL2 and analogues was observed.
