The Rat Gal4 Galectin-4 CLIA Kit (RTES00225) is a powerful tool for researchers looking to accurately detect and quantify Gal4 Galectin-4 levels in rat samples. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.Gal4 Galectin-4 is a key protein that plays a crucial role in various biological processes, including cell adhesion, migration, and signaling. Its dysregulation has been implicated in various diseases, making it a valuable biomarker for studying and developing potential therapies for these conditions.
With easy-to-follow protocols and all necessary reagents included, the Rat Gal4 Galectin-4 CLIA Kit (RTES00225) is a convenient and efficient solution for researchers looking to delve deeper into the role of Gal4 Galectin-4 in disease pathogenesis and potential therapeutic interventions.
Product Name:
Rat GAL4 (Galectin 4) CLIA Kit
SKU:
RTES00225
Target:
Rat GAL4 (Galectin 4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GAL4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GAL4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GAL4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GAL4. You can calculate the concentration of Rat GAL4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-100
100-113
89-103
Average (%)
95
106
96
1:4
Range (%)
85-96
101-112
87-99
Average (%)
90
106
92
1:8
Range (%)
89-100
88-103
97-111
Average (%)
94
94
104
1:16
Range (%)
90-103
99-111
97-113
Average (%)
97
106
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-100
93
EDTA plasma (n=5)
95-108
101
Cell culture media (n=5)
84-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
102.44
314.7
715.46
110.7
310.92
762.14
Standard deviation
10.73
26.06
63.1
12.63
26.27
71.79
C V (%)
10.47
8.28
8.82
11.41
8.45
9.42
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GAL4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GAL4 in samples. No significant cross-reactivity or interference between Rat GAL4 and analogues was observed.
