The Rat Gal9 (Galectin-9) CLIA Kit is specifically designed for the precise measurement of Galectin-9 levels in rat serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and accuracy, ensuring consistent and dependable results for a variety of research purposes.Galectin-9 is an important protein involved in immune regulation and inflammatory responses. Its role in various diseases, such as cancer, autoimmune disorders, and viral infections, makes it a valuable biomarker for studying disease mechanisms and potential therapeutic interventions.
With the Rat Gal9 CLIA Kit, researchers can reliably quantify Galectin-9 levels in rat samples, allowing for in-depth investigation into its biological functions and potential clinical implications. This kit provides a convenient and effective tool for advancing research in immunology, oncology, and other related fields.
Product Name:
Rat GAL9 (Galectin 9) CLIA Kit
SKU:
RTES00228
Target:
Rat GAL9 (Galectin 9)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
1.88 pg/mL
Detection range:
3.13-200 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GAL9. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GAL9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GAL9, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GAL9. You can calculate the concentration of Rat GAL9 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-99
103-118
98-115
Average (%)
91
109
105
1:4
Range (%)
86-98
100-111
101-118
Average (%)
93
105
108
1:8
Range (%)
90-102
97-113
99-112
Average (%)
96
103
107
1:16
Range (%)
98-116
101-118
85-99
Average (%)
107
108
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
99
EDTA plasma (n=5)
85-98
90
Cell culture media (n=5)
90-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.75
21.17
71.4
10.17
19.07
67.78
Standard deviation
0.95
2.53
7.52
1.12
1.62
5.26
C V (%)
9.74
11.95
10.53
11.01
8.5
7.76
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GAL9 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GAL9 in samples. No significant cross-reactivity or interference between Rat GAL9 and analogues was observed.
