The Rat GAL9 (Galectin 9) ELISA Kit is a specialized assay designed for the accurate quantification of Galectin 9 levels in rat samples. Galectin 9, a multifaceted protein, plays a crucial role in various biological processes, including immunomodulation, cell adhesion, and apoptosis. Studies have linked Galectin 9 to immune regulation, cancer progression, and autoimmune diseases, highlighting its significance in diverse physiological contexts. Precise measurement of Galectin 9 levels can offer valuable insights into its involvement in disease pathogenesis, immune responses, and tissue homeostasis.
The Rat GAL9 ELISA Kit provides researchers with a reliable tool to explore the functions and regulatory mechanisms of Galectin 9 in rat models, contributing to a deeper understanding of its implications in health and disease. With exceptional sensitivity and specificity, the Rat GAL9 ELISA Kit ensures accurate and reproducible results. Manufactured under rigorous quality control standards, this kit delivers robust performance and ease of use, making it an excellent choice for research in immunology, oncology, and various disease-related studies.
Product Name:
Rat GAL9 (Galectin 9) ELISA Kit
SKU:
AEES00404
Target:
Rat GAL9 (Galectin 9)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GAL9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GAL9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GAL9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat GAL9. You can calculate the concentration of Rat GAL9 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-113
90-100
96-112
Average (%)
105
95
102
1:4
Range (%)
87-104
85-97
82-94
Average (%)
95
92
88
1:8
Range (%)
87-99
87-98
82-94
Average (%)
93
92
89
1:16
Range (%)
89-103
86-98
86-97
Average (%)
95
92
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-104
97
EDTA plasma (n=5)
86-100
93
Cell culture media (n=5)
91-105
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.87
63.88
178.48
25.53
64.64
176.11
Standard deviation
1.56
2.71
8.39
1.59
3.34
8.82
C V (%)
5.81
4.24
4.7
6.23
5.17
5.01
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat GAL9 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GAL9 in samples. No significant cross-reactivity or interference between Rat GAL9 and analogues was observed.
