Based on the information provided, here is a product description:The Rat Gastrokine-1 (GKN1) ELISA Kit is a highly reliable and sensitive assay designed for the accurate detection of Gastrokine-1 levels in rat serum, plasma, and cell culture supernatants. Gastrokine-1 is a vital protein involved in gastrointestinal health and has been linked to various gastrointestinal disorders. This kit offers high specificity and reproducibility, providing researchers with dependable results for a wide range of research applications related to gastrointestinal health and disease.
By accurately measuring Gastrokine-1 levels, this ELISA kit can aid in understanding the role of GKN1 in gastrointestinal disorders and potentially lead to the development of new therapeutic strategies.Overall, the Rat Gastrokine-1 ELISA Kit is a valuable tool for researchers studying gastrointestinal health and disease, offering accurate and reliable measurements of Gastrokine-1 levels in rat samples.
Product Name:
Rat Gastrokine 1 (GKN1) ELISA Kit
Product Code:
RTDL00434
Size:
96 Assays
Target:
GKN1
Synonyms:
CA11, AMP18, BRICD1, FOV, Foveolin, 18 kDa antrum mucosa protein
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
