Rat GCSFR (Colony Stimulating Factor Receptor, Granulocyte) CLIA Kit
The Rat GCSFR (Granulocyte Colony Stimulating Factor Receptor) CLIA Kit is a comprehensive assay designed for the quantitative detection of GCSFR levels in rat serum, plasma, and tissue homogenates. This kit is highly sensitive and specific, providing accurate and reproducible results for a variety of research applications. GCSFR is a crucial receptor involved in granulocyte production and function, playing a key role in immune response and inflammation.
Dysregulation of GCSFR activity has been linked to various diseases such as leukemia, infection, and autoimmune disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions. With its superior performance and user-friendly protocol, the Rat GCSFR CLIA Kit is an essential tool for researchers looking to investigate the role of GCSFR in health and disease in rat models.
Product Name:
Rat GCSFR (Colony Stimulating Factor Receptor, Granulocyte) CLIA Kit
SKU:
RTES00147
Target:
Rat GCSFR (Colony Stimulating Factor Receptor, Granulocyte)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GCSFR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GCSFR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GCSFR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GCSFR. You can calculate the concentration of Rat GCSFR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-116
98-111
96-112
Average (%)
107
103
103
1:4
Range (%)
97-110
88-100
86-96
Average (%)
104
94
91
1:8
Range (%)
93-106
91-105
89-103
Average (%)
98
98
96
1:16
Range (%)
91-103
89-102
94-109
Average (%)
97
95
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-95
90
EDTA plasma (n=5)
91-104
99
Cell culture media (n=5)
91-105
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.9
77.41
178.09
25.77
69.92
168.6
Standard deviation
2.79
8.32
13.45
3.29
7.62
10.59
C V (%)
10.77
10.75
7.55
12.77
10.9
6.28
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GCSFR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GCSFR in samples. No significant cross-reactivity or interference between Rat GCSFR and analogues was observed.
