Rat GDF1 (Growth Differentiation Factor 1) CLIA Kit
The Rat GDF1 (Growth Differentiation Factor 1) CLIA Kit is specifically designed for the quantitative determination of GDF1 levels in rat serum, plasma, and cell culture supernatants. This kit provides accurate and reliable results with high sensitivity and specificity, making it an essential tool for research in a variety of fields.GDF1 is a critical factor involved in growth and differentiation processes, playing a key role in embryonic development and tissue repair. Dysregulation of GDF1 has been linked to various diseases, making it a valuable biomarker for studying and understanding these conditions.
With the Rat GDF1 CLIA Kit, researchers can accurately measure GDF1 levels in rat samples, allowing for in-depth analysis and investigation into the role of GDF1 in physiological and pathological processes. This kit provides a convenient and efficient way to study the functions and mechanisms of GDF1, furthering our understanding of its implications in various health conditions.
Product Name:
Rat GDF1 (Growth Differentiation Factor 1) CLIA Kit
SKU:
RTES00264
Target:
Rat GDF1 (Growth Differentiation Factor 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GDF1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GDF1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GDF1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GDF1. You can calculate the concentration of Rat GDF1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-103
92-105
95-108
Average (%)
95
98
102
1:4
Range (%)
88-103
97-110
96-109
Average (%)
95
102
102
1:8
Range (%)
98-109
104-120
97-107
Average (%)
103
110
102
1:16
Range (%)
93-108
102-120
100-117
Average (%)
99
109
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-105
99
EDTA plasma (n=5)
90-105
96
Cell culture media (n=5)
96-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
42.07
66.79
328.39
43.31
71.93
326.23
Standard deviation
4.16
7.31
36.09
5.28
7.57
37.32
C V (%)
9.89
10.94
10.99
12.19
10.52
11.44
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GDF1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GDF1 in samples. No significant cross-reactivity or interference between Rat GDF1 and analogues was observed.
