Rat GDF15 (Growth Differentiation Factor 15) CLIA Kit
The Rat GDF15 (Growth Differentiation Factor 15) CLIA Kit is specifically designed for the accurate measurement of GDF15 levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.GDF15 is a key regulatory protein involved in various physiological processes, including inflammation, metabolism, and cell differentiation. Dysregulation of GDF15 has been linked to several diseases, including cancer, cardiovascular disorders, and metabolic syndromes.
Therefore, accurate measurement of GDF15 levels is essential for understanding the pathogenesis of these conditions and identifying potential therapeutic targets.Overall, the Rat GDF15 CLIA Kit provides researchers with a reliable tool for studying the role of GDF15 in health and disease, contributing to advancements in biomedical research and drug development efforts.
Product Name:
Rat GDF15 (Growth Differentiation Factor 15) CLIA Kit
SKU:
RTES00269
Target:
Rat GDF15 (Growth Differentiation Factor 15)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GDF15. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GDF15 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GDF15, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GDF15. You can calculate the concentration of Rat GDF15 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-109
94-109
102-114
Average (%)
103
101
108
1:4
Range (%)
97-110
87-102
91-106
Average (%)
102
94
97
1:8
Range (%)
101-112
97-108
93-108
Average (%)
106
102
100
1:16
Range (%)
94-105
100-114
93-104
Average (%)
100
106
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-114
107
EDTA plasma (n=5)
93-104
99
Cell culture media (n=5)
84-99
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
42.51
112.17
341.59
43.79
122.2
341.1
Standard deviation
3.8
12.84
20.6
3.87
11.87
22.82
C V (%)
8.94
11.45
6.03
8.84
9.71
6.69
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GDF15 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GDF15 in samples. No significant cross-reactivity or interference between Rat GDF15 and analogues was observed.
