Rat GDF2 (Growth Differentiation Factor 2) CLIA Kit
The Rat GDF2 (Growth Differentiation Factor 2) CLIA Kit is a reliable and efficient tool for measuring GDF2 levels in rat samples. This kit offers high specificity and sensitivity, providing accurate and reproducible results for various research applications.GDF2 is a key factor in regulating cell growth and differentiation, playing a crucial role in embryonic development and tissue regeneration.
Dysregulation of GDF2 has been linked to various diseases and disorders, making it a valuable biomarker for studying and potentially treating these conditions.With the Rat GDF2 CLIA Kit, researchers can easily and effectively measure GDF2 levels in biological samples, aiding in the understanding of its role in physiological processes and disease pathogenesis.
Product Name:
Rat GDF2 (Growth Differentiation Factor 2) CLIA Kit
SKU:
RTES00265
Target:
Rat GDF2 (Growth Differentiation Factor 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GDF2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GDF2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GDF2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GDF2. You can calculate the concentration of Rat GDF2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-101
86-98
100-116
Average (%)
94
93
107
1:4
Range (%)
86-99
87-103
88-101
Average (%)
91
94
95
1:8
Range (%)
99-113
99-114
96-110
Average (%)
105
106
104
1:16
Range (%)
101-113
101-114
99-115
Average (%)
107
108
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-107
100
EDTA plasma (n=5)
85-98
92
Cell culture media (n=5)
97-109
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
204.89
449.16
1546.11
217.98
464.76
1655.27
Standard deviation
23.05
48.46
145.8
21.91
52.89
184.89
C V (%)
11.25
10.79
9.43
10.05
11.38
11.17
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GDF2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GDF2 in samples. No significant cross-reactivity or interference between Rat GDF2 and analogues was observed.
