Rat GFRa1 (Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1) ELISA Kit
The Rat GFRa1 (Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1) ELISA Kit is a specialized assay developed for the quantitative measurement of Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1 levels in various rat biological samples. GFRa1 is a critical component of the signaling pathway involved in cellular responses to neurotrophic factors, playing a key role in neuronal growth, survival, and differentiation processes. This ELISA kit provides researchers with a precise and reliable method to assess the levels of GFRa1, enabling a deeper understanding of its involvement in neural development, regeneration, and various neurological conditions.
By accurately quantifying GFRa1 expression, researchers can gain insights into its functions in neuroprotection, neuroregeneration, and potential therapeutic targets for neurodegenerative diseases. The Rat GFRa1 ELISA Kit from Assay Genie offers exceptional sensitivity and specificity, ensuring accurate and reproducible results in research applications. Manufactured under stringent quality control standards, this kit delivers robust performance and user-friendly protocols, making it a valuable tool for investigating the role of GFRa1 in rat models and advancing neuroscience research.
Product Name:
Rat GFRa1 (Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1) ELISA Kit
SKU:
AEES00562
Target:
Rat GFRa1 (Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GFRa1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GFRa1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GFRa1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat GFRa1. You can calculate the concentration of Rat GFRa1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-112
88-100
92-108
Average (%)
105
95
99
1:4
Range (%)
98-114
84-95
93-106
Average (%)
104
90
99
1:8
Range (%)
99-115
90-105
90-100
Average (%)
107
96
95
1:16
Range (%)
96-110
85-98
89-101
Average (%)
104
92
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
97
EDTA plasma (n=5)
88-102
95
Cell culture media (n=5)
87-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
98.05
289.24
787.56
89.47
277.95
721.8
Standard deviation
5.56
15.3
41.35
5.97
13.87
35.15
C V (%)
5.67
5.29
5.25
6.67
4.99
4.87
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat GFRa1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GFRa1 in samples. No significant cross-reactivity or interference between Rat GFRa1 and analogues was observed.
