The Rat GH (Growth Hormone) ELISA Kit is expertly crafted to facilitate the precise and quantitative analysis of growth hormone levels in various biological samples collected from rats. Growth hormone is a pivotal endocrine regulator essential for promoting growth, regulating metabolism, and influencing various physiological processes. Through accurate measurement of growth hormone concentrations, this ELISA kit enables in-depth exploration of growth-related mechanisms and physiological responses in rat models. Accurate quantification of growth hormone levels is crucial for understanding its role in growth and development, as well as its involvement in metabolic activities and overall health.
By employing this specialized ELISA assay, researchers can gain valuable insights into the dynamics of growth hormone concentrations under different experimental conditions, providing a deeper understanding of growth regulation and its implications in health and disease. The Rat GH ELISA Kit by Assay Genie delivers exceptional sensitivity and specificity, ensuring precise, reproducible, and dependable results. Rigorously manufactured under stringent quality controls, this kit ensures robust performance and user-friendly operation, making it an excellent choice for researchers investigating growth hormone biology and its impact on diverse physiological processes.
Product Name:
Rat GH (Growth Hormone) ELISA Kit
SKU:
AEES00519
Target:
Rat GH (Growth Hormone)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat GH. You can calculate the concentration of Rat GH in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-104
92-103
92-101
Average (%)
96
95
95
1:4
Range (%)
96-107
93-107
96-105
Average (%)
99
99
99
1:8
Range (%)
94-101
100-109
99-108
Average (%)
98
103
102
1:16
Range (%)
87-100
93-109
100-111
Average (%)
94
101
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-99
91
EDTA plasma (n=5)
90-102
98
Cell culture media (n=5)
92-106
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.43
10.28
41.61
4.92
12.26
43.56
Standard deviation
0.23
0.47
1.92
0.28
0.58
2.06
C V (%)
5.19
4.57
4.61
5.69
4.73
4.73
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat GH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GH in samples. No significant cross-reactivity or interference between Rat GH and analogues was observed.
