Rat GHRH (Growth Hormone Releasing Hormone) CLIA Kit
The Rat GHRH (Growth Hormone Releasing Hormone) CLIA Kit is a highly reliable and sensitive assay designed for the accurate detection of GHRH levels in rat samples such as serum, plasma, and cell culture supernatants. This kit offers high specificity, ensuring precise and reproducible results for a variety of research applications.GHRH is an important hormone that stimulates the release of growth hormone, playing a critical role in regulating growth, metabolism, and other physiological processes. Dysregulation of GHRH signaling is implicated in various disorders, including pituitary disorders, growth abnormalities, and metabolic conditions, making it a valuable biomarker for studying these conditions and developing therapeutic interventions.
Overall, the Rat GHRH CLIA Kit provides researchers with a powerful tool for studying the role of GHRH in physiological and pathological processes, offering accurate and reliable results to advance scientific understanding and potential clinical applications.
Product Name:
Rat GHRH (Growth Hormone Releasing Hormone) CLIA Kit
SKU:
RTES00272
Target:
Rat GHRH (Growth Hormone Releasing Hormone)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GHRH. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GHRH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GHRH, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GHRH. You can calculate the concentration of Rat GHRH in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-97
93-108
93-106
Average (%)
91
101
99
1:4
Range (%)
103-113
84-99
93-110
Average (%)
108
91
101
1:8
Range (%)
94-109
90-105
85-97
Average (%)
101
97
90
1:16
Range (%)
98-113
86-99
87-99
Average (%)
106
92
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-97
91
EDTA plasma (n=5)
100-117
108
Cell culture media (n=5)
99-113
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.34
58.89
207.4
24.18
63.49
206.49
Standard deviation
2.14
4.71
22.59
2.95
6.77
14.52
C V (%)
9.17
8.0
10.89
12.2
10.66
7.03
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GHRH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GHRH in samples. No significant cross-reactivity or interference between Rat GHRH and analogues was observed.
