The Rat GIP (Gastric Inhibitory Polypeptide) CLIA Kit is specifically designed to accurately measure GIP levels in rat serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, ensuring dependable and consistent results for a variety of research purposes.Gastric Inhibitory Polypeptide is a key hormone involved in regulating glucose metabolism and insulin secretion in response to food intake. Dysregulation of GIP levels has been linked to metabolic disorders such as obesity and diabetes, making it a valuable biomarker for studying these conditions and developing therapeutic interventions.
With the Rat GIP CLIA Kit, researchers can confidently assess GIP levels in rat samples, gaining valuable insights into the role of this hormone in metabolic health and disease. Trust in this kit for reliable and accurate measurements that drive impactful research outcomes.
Product Name:
Rat GIP (Gastric Inhibitory Polypeptide) CLIA Kit
SKU:
RTES00229
Target:
Rat GIP (Gastric Inhibitory Polypeptide)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GIP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GIP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GIP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GIP. You can calculate the concentration of Rat GIP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-106
89-102
90-101
Average (%)
99
95
95
1:4
Range (%)
90-105
90-102
93-106
Average (%)
96
97
100
1:8
Range (%)
96-111
101-115
96-111
Average (%)
103
109
101
1:16
Range (%)
95-107
98-110
100-114
Average (%)
101
104
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
93
EDTA plasma (n=5)
83-96
90
Cell culture media (n=5)
95-111
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
438.88
751.06
3716.85
409.79
679.71
3356.23
Standard deviation
40.86
79.99
236.76
43.48
69.33
305.08
C V (%)
9.31
10.65
6.37
10.61
10.2
9.09
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GIP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GIP in samples. No significant cross-reactivity or interference between Rat GIP and analogues was observed.
