The Rat GKN2 (Gastrokine 2) CLIA Kit is a reliable and accurate tool for the quantification of GKN2 levels in rat serum, plasma, and tissue lysates. This cutting-edge kit offers high sensitivity and specificity, ensuring precise and reproducible results for your research needs.Gastrokine 2, also known as GKN2, is a key protein involved in gastric mucosal protection and repair. It plays a crucial role in maintaining the integrity of the gastric mucosa and has been implicated in gastric diseases such as gastritis and gastric cancer.
By measuring GKN2 levels, researchers can gain valuable insights into the pathophysiology of these conditions and potentially identify new therapeutic targets.The Rat GKN2 CLIA Kit from Assay Genie provides an efficient and convenient solution for studying the role of Gastrokine 2 in gastric health and disease. With its easy-to-use format and reliable performance, this kit is a valuable asset for any laboratory conducting research on gastric biology and related disorders.
Product Name:
Rat GKN2 (Gastrokine 2) CLIA Kit
SKU:
RTES00232
Target:
Rat GKN2 (Gastrokine 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
1.88 pg/mL
Detection range:
3.13-200 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GKN2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GKN2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GKN2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GKN2. You can calculate the concentration of Rat GKN2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-99
102-118
100-116
Average (%)
91
109
107
1:4
Range (%)
94-107
89-102
97-111
Average (%)
101
95
103
1:8
Range (%)
96-113
104-120
98-114
Average (%)
104
110
105
1:16
Range (%)
93-108
89-105
100-112
Average (%)
101
96
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
83-97
90
EDTA plasma (n=5)
94-110
102
Cell culture media (n=5)
100-119
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
10.97
31.04
89.9
11.46
33.59
82.42
Standard deviation
1.37
3.55
9.12
1.39
2.89
6.19
C V (%)
12.49
11.44
10.14
12.13
8.6
7.51
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GKN2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GKN2 in samples. No significant cross-reactivity or interference between Rat GKN2 and analogues was observed.
