The Rat GLUT3 (Glucose Transporter 3) CLIA Kit is a highly reliable and sensitive assay designed for the accurate measurement of GLUT3 levels in rat samples. This kit is suitable for use with serum, plasma, and cell culture supernatants, providing researchers with precise and reproducible results for a variety of experimental applications.GLUT3 is a key transporter protein involved in glucose uptake and metabolism, playing a critical role in maintaining cellular energy balance. Dysregulation of GLUT3 expression has been linked to metabolic disorders, neurodegenerative diseases, and cancer, making it a valuable biomarker for investigating these conditions and developing potential therapeutic strategies.
With its high level of specificity and ease of use, the Rat GLUT3 CLIA Kit is an essential tool for researchers studying glucose metabolism and its impact on various disease processes in rat models. Trust in this kit to deliver accurate and dependable results for your research needs.
Product Name:
Rat GLUT3 (Glucose Transporter 3) CLIA Kit
SKU:
RTES00245
Target:
Rat GLUT3 (Glucose Transporter 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat Glut3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat Glut3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat Glut3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat Glut3. You can calculate the concentration of Rat Glut3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-106
99-115
84-95
Average (%)
97
105
90
1:4
Range (%)
101-119
102-117
88-102
Average (%)
109
110
95
1:8
Range (%)
85-99
93-105
99-112
Average (%)
92
99
106
1:16
Range (%)
93-104
85-97
100-115
Average (%)
99
91
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
102-117
107
EDTA plasma (n=5)
94-107
100
Cell culture media (n=5)
90-104
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
190.58
431.78
1914.11
172.25
468.43
2060.79
Standard deviation
20.7
31.74
124.42
15.59
51.48
220.3
C V (%)
10.86
7.35
6.5
9.05
10.99
10.69
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat Glut3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat Glut3 in samples. No significant cross-reactivity or interference between Rat Glut3 and analogues was observed.
