The Rat GLUT4 (Glucose Transporter 4) CLIA Kit is specifically designed for the quantitative detection of GLUT4 levels in rat samples. This kit boasts high sensitivity and specificity, providing accurate and reproducible results for a variety of research applications. GLUT4 is a key player in glucose metabolism, responsible for the uptake of glucose into cells. Dysregulation of GLUT4 expression and function has been linked to various metabolic disorders such as diabetes and obesity, making it a valuable target for studying these conditions and developing treatment strategies.
With its easy-to-use format and reliable performance, the Rat GLUT4 CLIA Kit from Assay Genie is a valuable tool for researchers looking to investigate the role of GLUT4 in metabolism and related diseases in rat models.
Product Name:
Rat GLUT4 (Glucose Transporter 4) CLIA Kit
SKU:
RTES00246
Target:
Rat GLUT4 (Glucose Transporter 4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat Glut4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat Glut4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat Glut4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat Glut4. You can calculate the concentration of Rat Glut4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-111
97-108
102-115
Average (%)
102
102
108
1:4
Range (%)
100-119
83-95
90-104
Average (%)
109
90
95
1:8
Range (%)
99-114
85-97
102-118
Average (%)
106
91
108
1:16
Range (%)
92-105
87-100
101-114
Average (%)
98
93
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-115
106
EDTA plasma (n=5)
96-111
101
Cell culture media (n=5)
86-97
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
405.19
808.13
3367.24
430.94
727.81
3612.82
Standard deviation
52.31
95.93
292.28
45.38
85.59
305.28
C V (%)
12.91
11.87
8.68
10.53
11.76
8.45
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat Glut4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat Glut4 in samples. No significant cross-reactivity or interference between Rat Glut4 and analogues was observed.
