Rat Glutathione S Transferase Theta 2 (GSTt2) ELISA Kit
The Rat Glutathione S-Transferase Theta 2 (GSTT2) ELISA Kit is a reliable and accurate tool for the detection of GSTT2 levels in rat serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, providing researchers with consistent and trustworthy results for a variety of research applications.GSTT2 is an important enzyme involved in the detoxification process, playing a key role in the metabolism of various xenobiotics and carcinogens.
Its aberrant expression has been linked to certain diseases and conditions, making it a valuable biomarker for studying these issues and developing potential treatment strategies.With the Rat GSTT2 ELISA Kit, researchers can confidently measure and analyze GSTT2 levels, furthering their understanding of its role in health and disease. Stay ahead in your research with this essential tool for GSTT2 detection.
Product Name:
Rat Glutathione S Transferase Theta 2 (GSTt2) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
82-102
92
EDTA plasma (n=5)
91-98
94
Heparin plasma (n=5)
86-101
93
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
