Rat Glycogen Phosphorylase, Muscle (PYGM) ELISA Kit
The Rat Glycogen Phosphorylase Muscle (PYGM) ELISA Kit is a highly sensitive and specific assay designed for accurate detection of PYGM levels in rat samples. This kit is ideal for researchers studying glycogen metabolism, muscle physiology, and metabolic disorders. PYGM is a key enzyme involved in glycogen breakdown in muscle tissue, playing a crucial role in energy production during exercise and maintaining glucose levels in the body.
Dysregulation of PYGM activity has been linked to conditions such as glycogen storage diseases and muscle disorders, making it a valuable biomarker for investigating these conditions.With its reliable and reproducible results, the Rat Glycogen Phosphorylase Muscle (PYGM) ELISA Kit is an essential tool for conducting in-depth research on muscle metabolism and related pathologies in rats.
Product Name:
Rat Glycogen Phosphorylase, Muscle (PYGM) ELISA Kit
Product Code:
RTDL00890
Size:
96 Assays
Target:
PYGM
Synonyms:
GPMM, Phosphorylase,Glycogen, McArdle Syndrome,Glycogen Storage Disease Type V, Myophosphorylase
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
