The Rat Glycoprotein-39 (GP39) Cartilage ELISA Kit is specifically designed for the accurate measurement of GP39 levels in rat serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, providing researchers with reliable and reproducible results for various research applications.GP39, also known as chondromodulin-I, is a glycoprotein primarily found in cartilage tissue. It plays a crucial role in regulating cartilage formation and maintenance, making it a key biomarker for studying cartilage-related diseases such as osteoarthritis and rheumatoid arthritis.
Understanding GP39 levels can help in developing targeted therapies for these conditions and advancing research in the field of musculoskeletal disorders.Overall, the Rat Glycoprotein-39 (GP39) Cartilage ELISA Kit is an essential tool for researchers looking to investigate the role of GP39 in cartilage biology and disease pathogenesis. With its high-performance features, this kit enables accurate quantification of GP39 levels, facilitating in-depth studies and breakthrough discoveries in the field of cartilage research.
Product Name:
Rat Glycoprotein 39, Cartilage (GP39) ELISA Kit
Product Code:
RTDL00449
Size:
96 Assays
Target:
GP39
Synonyms:
CHI3L1, CHI3-L1, YKL40, Chondrex, Chitinase 3-Like 1, Chondrocyte Protein YKL40, 39 kDa synovial protein
Detection Method:
Sandwich
Range:
3.12-200ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-98
94
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
